Application
Western blot analysis of PFTK1 Antibody (N-term P82) in mouse cerebellum tissue lysates (35ug/lane). PFTK1 (arrow) was detected using the purified Pab.Application
PFTK1 Antibody (N-term P82) (RB18842) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PFTK1 Antibody (N-term P82) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PFTK1 Antibody (N-term P82) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PFTK1 Antibody (N-term P82) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDK14; KIAA0834; PFTK1; Cyclin-dependent kinase 14; Cell division protein kinase 14; Serine/threonine-protein kinase PFTAIRE-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0834 PFTK1 |
|---|---|
| Alternative Names | CDK14 KIAA0834 PFTK1 Cyclin-dependent kinase 14 Cell division protein kinase 14 Serine/threonine-protein kinase PFTAIRE-1 |
| Molecular Weight(MW) | 53057 Da |
| Function | Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport |
| Tissue Specificity | Highly expressed in brain, pancreas, kidney, heart, testis and ovary. Also detected at lower levels in other tissues except in spleen and thymus where expression is barely detected |
| Cellular Localization | Cell membrane; Peripheral membrane protein. Cytoplasm. Nucleus. Note=Recruited to the cell membrane by CCNY |
| Entrez Gene | 5218 |
|---|
Application
Western blot analysis of PFTK1 Antibody (N-term P82) in mouse cerebellum tissue lysates (35ug/lane). PFTK1 (arrow) was detected using the purified Pab.
Application
PFTK1 Antibody (N-term P82) (RB18842) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PFTK1 Antibody (N-term P82) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PFTK1 Antibody (N-term P82) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
