Application
PFTK1 Antibody (N-term P82) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
PFTK1 Antibody (N-term P82) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PFTK1 Antibody (N-term P82) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of PFTK1 Antibody (N-term P82) in mouse cerebellum tissue lysates (35ug/lane). PFTK1 (arrow) was detected using the purified Pab.| Product Name | PFTK1 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PFTK1 (N-term P82)Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-149', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport. |
| Immunogen | KLH conjugated synthetic peptide between 74~104 amino acids from the N-terminal region of human PFTK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Gene Synonyms | KIAA0834 CDK14 PFTK1 Serine/threonine-protein kinase PFTAIRE-1 Serine/threonineprotein kinase PFTAIRE1 |
|---|---|
| Target | PFTK1 |
| Molecular Weight(MW) | 53.057 kDa (note) |
| Cellular Localization | Recruited to the cell membrane by CCNY. |
Application
PFTK1 Antibody (N-term P82) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
PFTK1 Antibody (N-term P82) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PFTK1 Antibody (N-term P82) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of PFTK1 Antibody (N-term P82) in mouse cerebellum tissue lysates (35ug/lane). PFTK1 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
