Application
Western blot analysis of PHAR4 Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). PHAR4 (arrow) was detected using the purified Pab.| Product Name | PHAR4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PHACTR4 Phosphatase and actin regulator 4 |
|---|---|
| Molecular Weight(MW) | 78211 Da |
| Function | Regulator of protein phosphatase 1 (PP1) required for neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. Acts as an activator of PP1 by interacting with PPP1CA and preventing phosphorylation of PPP1CA at 'Thr-320'. During neural tube closure, localizes to the ventral neural tube and activates PP1, leading to down-regulate cell proliferation within cranial neural tissue and the neural retina. Also acts as a regulator of migration of enteric neural crest cells (ENCCs) by activating PP1, leading to dephosphorylation and subsequent activation of cofilin (COF1 or COF2) and repression of the integrin signaling through the RHO/ROCK pathway (By similarity) |
| Tissue Specificity | This PHAR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543~572 amino acids from the C-terminal region of human PHAR4. |
| Cellular Localization | Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity) |
| Entrez Gene | 65979 |
|---|
Application
Western blot analysis of PHAR4 Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). PHAR4 (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
