Application
Western blot analysis of PHB2-Y248 in MDA-MB435 cell line lysates (35ug/lane).PHB2 (arrow) was detected using the purified Pab.Application
Western blot analysis of PHB2-Y248 in mouse kidney tissue lysates (35ug/lane). PHB2 (arrow) was detected using the purified Pab.Application
Western blot analysis of PHB2 (arrow) using PHB2 Antibody (Y248) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2) (Origene Technologies).Application
PHB2 Antibody (Y248) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHB2 Antibody (Y248) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PHB2 Antibody (Y248) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHB2; BAP; REA; Prohibitin-2; B-cell receptor-associated protein BAP37; D-prohibitin; Repressor of estrogen receptor activity |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BAP REA |
|---|---|
| Alternative Names | PHB2 BAP REA Prohibitin-2 B-cell receptor-associated protein BAP37 D-prohibitin Repressor of estrogen receptor activity |
| Molecular Weight(MW) | 33296 Da |
| Function | Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging |
| Tissue Specificity | This PHB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233~262 amino acids surrounding Y248 of human PHB2. |
| Cellular Localization | Mitochondrion inner membrane (By similarity). Cytoplasm (By similarity). Nucleus (By similarity) Note=Also cytoplasmic and nuclear (By similarity) |
Application
Western blot analysis of PHB2-Y248 in MDA-MB435 cell line lysates (35ug/lane).PHB2 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PHB2-Y248 in mouse kidney tissue lysates (35ug/lane). PHB2 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PHB2 (arrow) using PHB2 Antibody (Y248) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2) (Origene Technologies).
Application
PHB2 Antibody (Y248) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHB2 Antibody (Y248) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
