Application
Western blot analysis of anti-PHF2a/b (N-term) Pab in mouse kidney tissue lysate. PHF2a/b (N-term)(arrow) was detected using the purified Pab.| Product Name | PHF2a/b Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phf2; Kiaa0662; Lysine-specific demethylase PHF2; GRC5; PHD finger protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:250~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Kiaa0662 |
|---|---|
| Alternative Names | Phf2 Kiaa0662 Lysine-specific demethylase PHF2 GRC5 PHD finger protein 2 |
| Molecular Weight(MW) | 120814 Da |
| Function | Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA (By similarity) |
| Tissue Specificity | This PHF2a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~88 amino acids from the N-terminal region of human PHF2a/b. |
| Cellular Localization | Nucleus, nucleolus (By similarity). |
Application
Western blot analysis of anti-PHF2a/b (N-term) Pab in mouse kidney tissue lysate. PHF2a/b (N-term)(arrow) was detected using the purified Pab.| Application Notes | WB:1:250~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
