Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PHF2a/b (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
PHF2a/b Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the PHF2a/b antibody detected the PHF2a/b protein (arrow).| Product Name | PHF2a/b Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0662 |
|---|---|
| Alternative Names | PHF2 KIAA0662 Lysine-specific demethylase PHF2 GRC5 PHD finger protein 2 |
| Molecular Weight(MW) | 120775 Da |
| Function | Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA |
| Tissue Specificity | Widely expressed, including in liver (at protein level) |
| Cellular Localization | Nucleus, nucleolus. |
| Entrez Gene | 5253 |
|---|
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PHF2a/b (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
PHF2a/b Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the PHF2a/b antibody detected the PHF2a/b protein (arrow).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
