Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of PHKG2 (arrow) using rabbit polyclonal hPHKG2-G319 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHKG2 gene.Application
PHKG2 Antibody (G319) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PHKG2 antibody detected the PHKG2 protein (arrow).Application
PHKG2 Antibody (G319) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PHKG2 antibody detected the PHKG2 protein (arrow).| Product Name | PHKG2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PHKG2 Phosphorylase b kinase gamma catalytic chain testis/liver isoform PSK-C3 Phosphorylase kinase subunit gamma-2 Serine/threonine-protein kinase PHKG2 |
|---|---|
| Molecular Weight(MW) | 46442 Da |
| Function | Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM (By similarity) |
| Tissue Specificity | This PHKG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312~341 amino acids from the center region of human PHKG2. |
| Entrez Gene | 5261 |
|---|
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of PHKG2 (arrow) using rabbit polyclonal hPHKG2-G319 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHKG2 gene.
Application
PHKG2 Antibody (G319) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PHKG2 antibody detected the PHKG2 protein (arrow).
Application
PHKG2 Antibody (G319) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PHKG2 antibody detected the PHKG2 protein (arrow).| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
