Application
Western blot analysis of anti-PHLPP1 Antibody (N-term) in K562 cell line lysates (35ug/lane).PHLPP1 (arrow) was detected using the purified Pab (1:60dilution).| Product Name | PHLPP1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0606, PHLPP, PLEKHE1, SCOP |
|---|---|
| Alternative Names | PHLPP1 KIAA0606 PHLPP PLEKHE1 SCOP PH domain leucine-rich repeat-containing protein phosphatase 1 Pleckstrin homology domain-containing family E member 1 Suprachiasmatic nucleus circadian oscillatory protein |
| Molecular Weight(MW) | 184672 Da |
| Function | Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser- 657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1 Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. May act as a negative regulator of K-Ras signaling in membrane rafts |
| Tissue Specificity | In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78% of tested tumors (at protein level). Ubiquitously expressed in non-cancerous tissues |
| Cellular Localization | Cytoplasm. Membrane; Peripheral membrane protein. Nucleus. Note=In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells |
| Entrez Gene | 23239 |
|---|
Application
Western blot analysis of anti-PHLPP1 Antibody (N-term) in K562 cell line lysates (35ug/lane).PHLPP1 (arrow) was detected using the purified Pab (1:60dilution).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
