Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Mst1 (pT183)/ Mst2 (pT180) RabMAb (Cat. #AJ1603a), 1:2000 dilution (A,B); Mst1 (1941-1), 1:5000 dilution (C,D) Cells were either (A,C) untreated or (B,D) treated with Calvculin A.| Product Name | Phospho Mst1 (pT183)/Mst2 (pT180) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T180 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1467Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KRS2, MST1 |
|---|---|
| Alternative Names | STK4 MST1 Serine/threonine-protein kinase 4 Mammalian STE20-like protein kinase 1 Serine/threonine-protein kinase Krs-2 |
| Molecular Weight(MW) | 55630 Da |
| Function | Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2 Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T) Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1 Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes |
| Tissue Specificity | Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Ubiquitously expressed |
| Cellular Localization | Cytoplasm. Nucleus. Note=The caspase-cleaved form cycles between the nucleus and cytoplasm |
| Entrez Gene | 6789 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Mst1 (pT183)/ Mst2 (pT180) RabMAb (Cat. #AJ1603a), 1:2000 dilution (A,B); Mst1 (1941-1), 1:5000 dilution (C,D) Cells were either (A,C) untreated or (B,D) treated with Calvculin A.| Application Notes | WB:1:1000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
