Application
Dot blot analysis of anti-Phospho- P21CIP1-S146 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
The anti-Phospho- P21CIP1-S146 Pab is used in Western blot to detect Phospho- P21CIP1-S146 in, from left to right, Hela, Y79, T47D, and 293 tissue lysates.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma| Product Name | Phospho- P21CIP1-S146 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S146 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBWBIHC |
| DB:1:500 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WA |
|---|---|
| Alternative Names | CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 Cyclin-dependent kinase inhibitor 1 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 p21 |
| Molecular Weight(MW) | 18119 Da |
| Function | May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex |
| Tissue Specificity | Expressed in all adult tissues, with 5-fold lower levels observed in the brain |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 1026 |
|---|
Application
Dot blot analysis of anti-Phospho- P21CIP1-S146 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
The anti-Phospho- P21CIP1-S146 Pab is used in Western blot to detect Phospho- P21CIP1-S146 in, from left to right, Hela, Y79, T47D, and 293 tissue lysates.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma| Application Notes | DB:1:500 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
