Application
Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5min), using 4E-BP1 Antibody (T45) (AE1002a, Lane1 and 2) and Phospho-4E-BP1-T45 Antibody (#AE1002b, Lane 3 and 4).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-4E-BP1-T45 Antibody (#AE1002b).| Product Name | Phospho-4E-BP1-T45 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T45 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Phosphorylated heat- and acid-stable protein regulated by insulin 1 PHAS-I Eukaryotic translation initiation factor 4E-binding protein 1 eIF4E-binding protein 1 4E-BP1 |
|---|---|
| Molecular Weight(MW) | 12580 Da |
| Function | Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways |
| Tissue Specificity | The antibody was affinity-purified from rabbit antiserum using epitope-specific phosphopeptide column, and the antibody against non-phosphopeptide was removed using non-phosphopeptide column corresponding to the phosphorylation site. |
| Entrez Gene | 1978 |
|---|
Application
Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5min), using 4E-BP1 Antibody (T45) (AE1002a, Lane1 and 2) and Phospho-4E-BP1-T45 Antibody (#AE1002b, Lane 3 and 4).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-4E-BP1-T45 Antibody (#AE1002b).| Application Notes | WB:1:500~1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
