Application
Dot blot analysis of Phospho-bcl-2-S70 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.Application
Western blot analysis of extracts from Jurkat cells,untreated or treated with paclitaxel,using phospho-Bcl2(Ser70)(left) or Bcl2 antibody(right).| Product Name | Phospho-bcl-2-S70 |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S70 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBWB |
| DB:1:500 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BCL2 Apoptosis regulator Bcl-2 |
|---|---|
| Molecular Weight(MW) | 26266 Da |
| Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) |
| Tissue Specificity | Expressed in a variety of tissues. |
| Cellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein Endoplasmic reticulum membrane; Single-pass membrane protein |
| Entrez Gene | 596 |
|---|---|
| Nucleotide Accession | NP_000624.2 |
Application
Dot blot analysis of Phospho-bcl-2-S70 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Application
Western blot analysis of extracts from Jurkat cells,untreated or treated with paclitaxel,using phospho-Bcl2(Ser70)(left) or Bcl2 antibody(right).| Application Notes | DB:1:500 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
