Application
The CDK7-T170 Pab is used in Western blot to detect CDK7-T170 in Ramos tissue lysate.Application
Dot blot analysis of Phospho-Cdk7-T170 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-CDK7-T170 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T170 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBDB |
| WB:1:100~500 DB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAK, CAK1, CDKN7, MO15, STK1 |
|---|---|
| Alternative Names | CDK7 CAK CAK1 CDKN7 MO15 STK1 Cyclin-dependent kinase 7 39 kDa protein kinase CDK-activating kinase 1 Cell division protein kinase 7 Serine/threonine-protein kinase 1 TFIIH basal transcription factor complex kinase subunit |
| Molecular Weight(MW) | 39038 Da |
| Function | Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin- dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Colocalizes with PRKCI in the cytoplasm and nucleus Translocates from the nucleus to cytoplasm and perinuclear region in response to DNA-bound peptides |
| Entrez Gene | 1022 |
|---|
Application
The CDK7-T170 Pab is used in Western blot to detect CDK7-T170 in Ramos tissue lysate.
Application
Dot blot analysis of Phospho-Cdk7-T170 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | WB:1:100~500 DB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
