Application
Western blot analysis of anti-Phospho-cJun-pS73 Pab in CEM cell line tissue lysate (35ug/lane).Phospho-cJun-pS73(arrow) was detected using the purified Pab (1:60 dilution).| Product Name | Phospho-cJun-S73 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S73 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | JUN Transcription factor AP-1 Activator protein 1 Proto-oncogene c-Jun V-jun avian sarcoma virus 17 oncogene homolog p39 |
|---|---|
| Molecular Weight(MW) | 35676 Da |
| Function | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation |
| Tissue Specificity | This Phospho-cJun-S73 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S73 of human cJun. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 3725 |
|---|
Application
Western blot analysis of anti-Phospho-cJun-pS73 Pab in CEM cell line tissue lysate (35ug/lane).Phospho-cJun-pS73(arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
