Application
Dot blot analysis of anti-Phospho-CLASP1-pT656 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-CLASP1-pT656 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T656 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0622, MAST1 |
|---|---|
| Alternative Names | CLASP1 KIAA0622 MAST1 CLIP-associating protein 1 Cytoplasmic linker-associated protein 1 Multiple asters homolog 1 Protein Orbit homolog 1 |
| Molecular Weight(MW) | 169451 Da |
| Function | Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle |
| Tissue Specificity | This Phospho-CLASP1-pT656 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 643~672 amino acids surrounding T656 of human CLASP1. |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle. Golgi apparatus, trans-Golgi network (Probable). Note=Localizes to microtubule plus ends Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella Localizes to the cell cortex and this requires ERC1 and PHLDB2 |
| Entrez Gene | 23332 |
|---|
Application
Dot blot analysis of anti-Phospho-CLASP1-pT656 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
