Application
Western blot analysis of anti-Phospho-CREB-pS133 Pab in CEM cell line tissue lysate (35ug/lane).Phospho-CREB-pS133(arrow) was detected using the purified Pab (1:40 dilution).| Product Name | Phospho-CREB-S133 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S133 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CREB1 Cyclic AMP-responsive element-binding protein 1 |
|---|---|
| Molecular Weight(MW) | 36688 Da |
| Function | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells |
| Tissue Specificity | This Phospho-CREB-S133 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S133 of human CREB. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 1385 |
|---|
Application
Western blot analysis of anti-Phospho-CREB-pS133 Pab in CEM cell line tissue lysate (35ug/lane).Phospho-CREB-pS133(arrow) was detected using the purified Pab (1:40 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
