Application
Western blot analysis of extracts from HeLa cells usingCREB Antibody (S133) (#AE1010b, Lane 1 and 2) andPhospho-CREB-S133 Antibody (#AE1010d, Lane 3 and4).Application
Immunohistochemical analysis of paraffin-embeddedhuman breast carcinoma tissue using Phospho-CREB-S133 Antibody (#AE1010d).| Product Name | Phospho-CREB-S133 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S133 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Cyclic AMP-responsive element-binding protein 1 cAMP-responsive element-binding protein 1 CREB-1 |
|---|---|
| Molecular Weight(MW) | 36688 Da |
| Function | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells |
| Tissue Specificity | The antibody was affinity-purified from rabbit antiserum using epitope-specific phosphopeptide column, and the antibody against non-phosphopeptide was removed using non-phosphopeptide column corresponding to the phosphorylation site. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 1385 |
|---|
Application
Western blot analysis of extracts from HeLa cells usingCREB Antibody (S133) (#AE1010b, Lane 1 and 2) andPhospho-CREB-S133 Antibody (#AE1010d, Lane 3 and4).
Application
Immunohistochemical analysis of paraffin-embeddedhuman breast carcinoma tissue using Phospho-CREB-S133 Antibody (#AE1010d).| Application Notes | WB:1:500~1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
