Application
Western blot analysis of extract from NIH/3T3 cellsuntreated or treated with 10% serum (15min), using EIF4E Antibody (S209) (#AE1011a, Line 1 and 2) and Phospho-EIF4E-S209 Antibody (#AE1011b, Line 3 and 4).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Phospho-EIF4E-S209 Antibody (#AE1011b).| Product Name | Phospho-EIF4E-S209 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S209 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EIF4EL1, EIF4F |
|---|---|
| Alternative Names | eIF-4F 25 kDa subunit mRNA cap-binding protein Eukaryotic translation initiation factor 4E eIF-4E eIF4E |
| Molecular Weight(MW) | 25097 Da |
| Function | Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap |
| Tissue Specificity | The antibody was affinity-purified from rabbit antiserum using epitope-specific phosphopeptide column, and the antibody against non-phosphopeptide was removed using non-phosphopeptide column corresponding to the phosphorylation site. |
| Cellular Localization | Cytoplasm, P-body. |
| Entrez Gene | 1977 |
|---|
Application
Western blot analysis of extract from NIH/3T3 cellsuntreated or treated with 10% serum (15min), using EIF4E Antibody (S209) (#AE1011a, Line 1 and 2) and Phospho-EIF4E-S209 Antibody (#AE1011b, Line 3 and 4).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Phospho-EIF4E-S209 Antibody (#AE1011b).| Application Notes | WB:1:500~1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
