Application
A. Western blot analysis on 293 cell lysates using anti-Phospho-eIF4E (pS209) RabMAb (Cat. #AJ1255c), 1:10,000 dilution. Cells were either (A) untreated (B) treated with APApplication
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Phospho-eIF4E (pS209) RabMAb (Cat. #AJ1255c).| Product Name | eIF-4E Antibody Phospho (pS209) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S209 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2151Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EIF4EL1, EIF4F |
|---|---|
| Alternative Names | EIF4E EIF4EL1 EIF4F Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit |
| Molecular Weight(MW) | 25097 Da |
| Function | Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap |
| Tissue Specificity | A synthetic peptide corresponding to residues surrounding Serine 209 of human eIF4E was used as an immunogen. |
| Cellular Localization | Cytoplasm, P-body. |
| Entrez Gene | 1977 |
|---|
Application
A. Western blot analysis on 293 cell lysates using anti-Phospho-eIF4E (pS209) RabMAb (Cat. #AJ1255c), 1:10,000 dilution. Cells were either (A) untreated (B) treated with AP
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Phospho-eIF4E (pS209) RabMAb (Cat. #AJ1255c).| Application Notes | WB:1:1000~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
