Application
Dot blot analysis of Phospho-ELK1-S353 polyclonal antibody (Cat# AP3309a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.| Product Name | Phospho-ELK1-S383 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S383 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ELK1 ETS domain-containing protein Elk-1 |
|---|---|
| Molecular Weight(MW) | 44888 Da |
| Function | Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element |
| Tissue Specificity | Lung and testis. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2002 |
|---|
Application
Dot blot analysis of Phospho-ELK1-S353 polyclonal antibody (Cat# AP3309a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.| Application Notes | DB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
