Application
Dot blot analysis of anti-Phospho-eNos-S1177 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
Confocal immunofluorescent analysis of Phospho-eNos-S1177 Antibody (Cat#AP3665a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | Phospho-eNos-S1177 |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S1177 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBIF |
| DB:1:500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NOS3 Nitric oxide synthase endothelial Constitutive NOS EC-NOS Endothelial NOS NOS type III |
|---|---|
| Molecular Weight(MW) | 133289 Da |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets |
| Tissue Specificity | Platelets, placenta, liver and kidney. |
| Cellular Localization | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity |
| Entrez Gene | 4846 |
|---|
Application
Dot blot analysis of anti-Phospho-eNos-S1177 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
Confocal immunofluorescent analysis of Phospho-eNos-S1177 Antibody (Cat#AP3665a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | DB:1:500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.17% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
