Application
Dot blot analysis of anti-FAS-pY232 Phospho-specific Pab (RB12781) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-FAS-Y232 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Y232 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APT1, FAS1, TNFRSF6 |
|---|---|
| Alternative Names | FAS APT1 FAS1 TNFRSF6 Tumor necrosis factor receptor superfamily member 6 Apo-1 antigen Apoptosis-mediating surface antigen FAS FASLG receptor CD_antigen=CD95 Flags: Precursor |
| Molecular Weight(MW) | 37732 Da |
| Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro) |
| Tissue Specificity | Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6 |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 3: Secreted. Isoform 5: Secreted. |
| Entrez Gene | 355 |
|---|
Application
Dot blot analysis of anti-FAS-pY232 Phospho-specific Pab (RB12781) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
