Application
Dot blot analysis of Phospho-Gab1-Y659 polyclonal antibody (Cat. AP3302b) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration were 0.5ug per ml.| Product Name | Phospho-Gab1-Y659 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Y659 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GAB1 GRB2-associated-binding protein 1 GRB2-associated binder 1 Growth factor receptor bound protein 2-associated protein 1 |
|---|---|
| Molecular Weight(MW) | 76616 Da |
| Function | Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR) |
| Tissue Specificity | This Phospho-Gab1-Y659 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 645~675 amino acids surrounding Y659 of human Gab1. |
| Entrez Gene | 2549 |
|---|
Application
Dot blot analysis of Phospho-Gab1-Y659 polyclonal antibody (Cat. AP3302b) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration were 0.5ug per ml.| Application Notes | DB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
