Application
Dot blot analysis of anti-Phospho-GIT1-pS388 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-GIT1- pS388 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S388 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GIT1 ARF GTPase-activating protein GIT1 Cool-associated and tyrosine-phosphorylated protein 1 G protein-coupled receptor kinase-interactor 1 GRK-interacting protein 1 |
|---|---|
| Molecular Weight(MW) | 84341 Da |
| Function | GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine- phosphorylated paxillin in cytoplasmic complexes |
| Tissue Specificity | This Phospho-GIT1-pS388 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between amino acids 372-401 surrounding S388 of human GIT1. |
| Cellular Localization | Cytoplasm. Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin |
| Entrez Gene | 28964 |
|---|---|
| Nucleotide Accession | NP_054749.2 NP_001078923.1 |
Application
Dot blot analysis of anti-Phospho-GIT1-pS388 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
