Application
Western blot analysis of extracts from HT-29 cellsuntreated or treated with UV (20min), using HDAC2 Antibody (S394) (#AE1014a, Lane 1 and 2) and Phospho-HDAC2-S394 Antibody (#AE1014b, Lane 3 and 4).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-HDAC2-S394 Antibody (#AE1014b).Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining using Phospho-HDAC2-S394 Antibody (#AE1014b).| Product Name | Phospho-HDAC2-S394 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S394 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500~1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Histone deacetylase 2 HD2 |
|---|---|
| Molecular Weight(MW) | 55364 Da |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development |
| Tissue Specificity | Widely expressed; lower levels in brain and lung |
| Cellular Localization | Nucleus. |
| Entrez Gene | 3066 |
|---|
Application
Western blot analysis of extracts from HT-29 cellsuntreated or treated with UV (20min), using HDAC2 Antibody (S394) (#AE1014a, Lane 1 and 2) and Phospho-HDAC2-S394 Antibody (#AE1014b, Lane 3 and 4).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-HDAC2-S394 Antibody (#AE1014b).
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining using Phospho-HDAC2-S394 Antibody (#AE1014b).| Application Notes | WB:1:500~1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
