Application
The anti-Phospho-IL1R-Y496 Pab is used in Western blot to detect Phospho-IL1R-Y496 in mouse liver tissue lysateApplication
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Phospho-IL1R-Y496 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Y496 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IL1R, IL1RA, IL1RT1 |
|---|---|
| Alternative Names | IL1R1 IL1R IL1RA IL1RT1 Interleukin-1 receptor type 1 CD121 antigen-like family member A Interleukin-1 receptor alpha Interleukin-1 receptor type I p80 CD_antigen=CD121a Interleukin-1 receptor type 1 membrane form Interleukin-1 receptor type 1 |
| Molecular Weight(MW) | 65402 Da |
| Function | Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex |
| Tissue Specificity | This Phospho-IL1R-Y496 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y496 of human IL1R. |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Cell membrane (Probable). Secreted |
| Entrez Gene | 3554 |
|---|
Application
The anti-Phospho-IL1R-Y496 Pab is used in Western blot to detect Phospho-IL1R-Y496 in mouse liver tissue lysate
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
