Application
Dot blot analysis of anti-Phospho-IRS1-pY896 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
Confocal immunofluorescent analysis of Phospho-IRS1-pY896 Antibody(Cat#AP3555a) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | Phospho-IRS1-pY896 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Y896 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBIF |
| DB:1:500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | IRS1 Insulin receptor substrate 1 |
|---|---|
| Molecular Weight(MW) | 131591 Da |
| Function | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2 Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity) |
| Tissue Specificity | This Phospho-IRS1-pY896 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 878~908 amino acids surrounding Y896 of human IRS1. |
| Entrez Gene | 3667 |
|---|
Application
Dot blot analysis of anti-Phospho-IRS1-pY896 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
Confocal immunofluorescent analysis of Phospho-IRS1-pY896 Antibody(Cat#AP3555a) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | DB:1:500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
