Application
Dot blot analysis of anti-Phospho-JMJD2B-pT305 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-JMJD2B-pT305 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T305 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JHDM3B, JMJD2B, KIAA0876 |
|---|---|
| Alternative Names | KDM4B JHDM3B JMJD2B KIAA0876 Lysine-specific demethylase 4B JmjC domain-containing histone demethylation protein 3B Jumonji domain-containing protein 2B |
| Molecular Weight(MW) | 121897 Da |
| Function | Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate |
| Tissue Specificity | This Phospho-JMJD2B-pT305 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 287~316 amino acids surrounding pT305 of human JMJD2B. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 23030 |
|---|
Application
Dot blot analysis of anti-Phospho-JMJD2B-pT305 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
