Application
A. Western blot analysis on Hek293 cell lysates using anti-JNK3 Phospho (pS223) RabMAb (Cat. #AJ1424a), 1:20,000 dilution. Cells were either (A) untreated (B) treated with UV.| Product Name | Phospho-JNK1 (pY185) / JNK2 (pY185) /JNK3 (pY223) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Y223 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1597Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JNK3, JNK3A, PRKM10, SAPK1B |
|---|---|
| Alternative Names | MAPK10 JNK3 JNK3A PRKM10 Mitogen-activated protein kinase 10 Stress-activated protein kinase JNK3 c-Jun N-terminal kinase 3 MAP kinase p49 3F12 |
| Molecular Weight(MW) | 52585 Da |
| Function | Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress- activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the beta-amyloid precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons |
| Tissue Specificity | Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney |
| Cellular Localization | Cytoplasm. Membrane; Lipid-anchor. Nucleus. Mitochondrion. Note=Palmitoylation regulates MAPK10 trafficking to cytoskeleton. Recruited to the mitochondria in the presence of SARM1 (By similarity) |
| Entrez Gene | 5602 |
|---|
Application
A. Western blot analysis on Hek293 cell lysates using anti-JNK3 Phospho (pS223) RabMAb (Cat. #AJ1424a), 1:20,000 dilution. Cells were either (A) untreated (B) treated with UV.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
