Application
Fluorescent image of U251 cells stained with phospho-LC3A-S3 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP36413a phospho-LC3A-S3 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Phospho- LC3A-S3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Application
Dot blot analysis of anti-Phospho-APG8a (MAP1LC3A)-pS3 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-LC3A-S3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFDB |
| IF:1:200 DB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MAP1LC3A Microtubule-associated proteins 1A/1B light chain 3A Autophagy-related protein LC3 A Autophagy-related ubiquitin-like modifier LC3 A MAP1 light chain 3-like protein 1 MAP1A/MAP1B light chain 3 A Microtubule-associated protein 1 light chain 3 |
|---|---|
| Molecular Weight(MW) | 14272 Da |
| Function | Involved in formation of autophagosomal vacuoles (autophagosomes) |
| Tissue Specificity | Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes |
| Cellular Localization | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome. Note=LC3-II binds to the autophagic membranes |
| Entrez Gene | 84557 |
|---|
Application
Fluorescent image of U251 cells stained with phospho-LC3A-S3 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP36413a phospho-LC3A-S3 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Phospho- LC3A-S3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Application
Dot blot analysis of anti-Phospho-APG8a (MAP1LC3A)-pS3 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | IF:1:200 DB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
