Application
Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates; S12A = LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDaApplication
Dot blot analysis of Phospho-LC3 (APG8a) - S12 Antibody and Nonphospho-LC3 (APG8a) Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h was probed with AP3301a. The data shows that treatment with rapamycin showed no significant change in level of LC3.Application
Immunoblots of SH-SY5Y cells treated with MPP+ for 24h was probed with AP3301a. The data shows that treatment with MPP+ showed no significant change in level of LC3.Application
Something like SH-SY5Y cells expressing GFP-LC3-WT or-S12D treated with rapamycin or vehicle for 1h.| Product Name | Phospho-LC3C-S12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB,DB |
| WB:1:100~500 DB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MAP1LC3A Microtubule-associated proteins 1A/1B light chain 3A Autophagy-related protein LC3 A Autophagy-related ubiquitin-like modifier LC3 A MAP1 light chain 3-like protein 1 MAP1A/MAP1B light chain 3 A Microtubule-associated protein 1 light chain 3 |
|---|---|
| Molecular Weight(MW) | 14272 Da |
| Function | Involved in formation of autophagosomal vacuoles (autophagosomes) |
| Tissue Specificity | Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes |
| Cellular Localization | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome. Note=LC3-II binds to the autophagic membranes |
| Entrez Gene | 84557 |
|---|
Application
Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates; S12A = LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa
Application
Dot blot analysis of Phospho-LC3 (APG8a) - S12 Antibody and Nonphospho-LC3 (APG8a) Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h was probed with AP3301a. The data shows that treatment with rapamycin showed no significant change in level of LC3.
Application
Immunoblots of SH-SY5Y cells treated with MPP+ for 24h was probed with AP3301a. The data shows that treatment with MPP+ showed no significant change in level of LC3.
Application
Something like SH-SY5Y cells expressing GFP-LC3-WT or-S12D treated with rapamycin or vehicle for 1h.| Application Notes | WB:1:100~500 DB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
