Application
Dot blot analysis of Phospho-LC3 (APG8c) - S9 Antibody (Cat# AP3457a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-LC3C-S9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MAP1LC3C Microtubule-associated proteins 1A/1B light chain 3C Autophagy-related protein LC3 C Autophagy-related ubiquitin-like modifier LC3 C MAP1 light chain 3-like protein 3 MAP1A/MAP1B light chain 3 C Microtubule-associated protein 1 light chain 3 |
|---|---|
| Molecular Weight(MW) | 16852 Da |
| Function | Probably involved in formation of autophagosomal vacuoles (autophagosomes) (By similarity) |
| Tissue Specificity | Most abundant in placenta, lung and ovary. |
| Cellular Localization | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes |
| Entrez Gene | 440738 |
|---|
Application
Dot blot analysis of Phospho-LC3 (APG8c) - S9 Antibody (Cat# AP3457a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
