Application
Dot blot analysis of Phospho-MAP2K3-S189 polyclonal antibody (Cat# AP3342a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.| Product Name | Phospho-MAP2K3-S189 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S189 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MEK3, MKK3, PRKMK3, SKK2 |
|---|---|
| Alternative Names | MAP2K3 MEK3 MKK3 PRKMK3 SKK2 Dual specificity mitogen-activated protein kinase kinase 3 MAPK/ERK kinase 3 Stress-activated protein kinase kinase 2 |
| Molecular Weight(MW) | 39318 Da |
| Function | Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38 |
| Tissue Specificity | Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues |
| Entrez Gene | 5606 |
|---|
Application
Dot blot analysis of Phospho-MAP2K3-S189 polyclonal antibody (Cat# AP3342a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.| Application Notes | DB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
