Application
Dot blot analysis of Phospho-MAP3K1-T1383 Pab (Cat.AP3321a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration were 0.5ug per ml.| Product Name | Phospho-MAP3K1-T1383 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T1383 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:0.388888888889 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAPKKK1, MEKK, MEKK1 |
|---|---|
| Alternative Names | MAP3K1 MAPKKK1 MEKK MEKK1 Mitogen-activated protein kinase kinase kinase 1 MAPK/ERK kinase kinase 1 |
| Molecular Weight(MW) | 164470 Da |
| Function | Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway |
| Tissue Specificity | This Phospho-MAP3K1-T1383 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T1383 of human MAP3K1. |
| Entrez Gene | 4214 |
|---|
Application
Dot blot analysis of Phospho-MAP3K1-T1383 Pab (Cat.AP3321a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration were 0.5ug per ml.| Application Notes | DB:0.388888888889 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
