Application
Dot blot analysis of anti-Phospho-ERK5-pT218 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-MAPK7-pT218 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T218 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BMK1, ERK5, PRKM7 |
|---|---|
| Alternative Names | MAPK7 BMK1 ERK5 PRKM7 Mitogen-activated protein kinase 7 Big MAP kinase 1 Extracellular signal-regulated kinase 5 |
| Molecular Weight(MW) | 88386 Da |
| Function | Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras- independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression |
| Tissue Specificity | Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocates to the nucleus upon activation |
| Entrez Gene | 5598 |
|---|
Application
Dot blot analysis of anti-Phospho-ERK5-pT218 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
