Application
Dot blot analysis of anti-MAP4K4-pS801 Phospho-specific Pab (RB11324) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-MAP4K4-S801 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S801 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HGK, KIAA0687, NIK |
|---|---|
| Alternative Names | MAP4K4 HGK KIAA0687 NIK Mitogen-activated protein kinase kinase kinase kinase 4 HPK/GCK-like kinase HGK MAPK/ERK kinase kinase kinase 4 Nck-interacting kinase |
| Molecular Weight(MW) | 142101 Da |
| Function | Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha Appears to act upstream of the JUN N-terminal pathway Phosphorylates SMAD1 on Thr-322 |
| Tissue Specificity | Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 9448 |
|---|
Application
Dot blot analysis of anti-MAP4K4-pS801 Phospho-specific Pab (RB11324) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
