A. Western blot analysis on HeLa cell lysates using anti-Phospho- Mst4 (pT178) RabMAb (Cat. #AJ1605a), 1:2000 dilution. Cells were either (A) untreated (B) treated with calyculin A.
A phospho specific peptide corresponding to residues surrounding threonine 178 of human MST4, theonine 190 of human MSt 3 and threonine 174 of human STK25.
Cellular Localization
Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Localizes to the Golgi apparatus
A. Western blot analysis on HeLa cell lysates using anti-Phospho- Mst4 (pT178) RabMAb (Cat. #AJ1605a), 1:2000 dilution. Cells were either (A) untreated (B) treated with calyculin A.
Application Notes
WB:1:1000~5000
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.