Application
Dot blot analysis of Phospho-MYC-S373 Pab (Cat.AP3326a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-MYC-S373 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S373 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHE39 |
|---|---|
| Alternative Names | MYC BHLHE39 Myc proto-oncogene protein Class E basic helix-loop-helix protein 39 Proto-oncogene c-Myc Transcription factor p64 |
| Molecular Weight(MW) | 48804 Da |
| Function | Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes |
| Tissue Specificity | This Phospho-MYC-S373 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S373 of human MYC. |
| Cellular Localization | Nucleus, nucleoplasm. Nucleus, nucleolus. |
| Entrez Gene | 4609 |
|---|
Application
Dot blot analysis of Phospho-MYC-S373 Pab (Cat.AP3326a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
