Application
A. Western blot analysis on A431 cell lysate using anti-Phospho-c-Myc (pT58/pS62) RabMAb (Cat. #AJ1192c), dilution 1:1,000. Cell lysates were either (1) untreated or (2) treated with TPA.Application
B. Immunofluorescent staining of A4321 cells using anti-phospho-c-Myc (T58/S62) RabMAb (Cat. #AJ1192c).| Product Name | c-Myc Antibody Phospho (pT58/pS62) |
|---|---|
| Antibody Type | Tags Antibodies |
| Antigen Alias | MYC, Myc proto-oncogene protein, c-Myc;Transcription factor p64 |
| Modification | p-T58/p-S62 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E203 |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHE39 |
|---|---|
| Alternative Names | MYC Myc proto-oncogene protein c-Myc Transcription factor p64 |
| Molecular Weight(MW) | 48804 Da |
| Function | Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequence homology. |
| Cellular Localization | Nucleus, nucleoplasm. Nucleus, nucleolus. |
| Entrez Gene | 4609 |
|---|
Application
A. Western blot analysis on A431 cell lysate using anti-Phospho-c-Myc (pT58/pS62) RabMAb (Cat. #AJ1192c), dilution 1:1,000. Cell lysates were either (1) untreated or (2) treated with TPA.
Application
B. Immunofluorescent staining of A4321 cells using anti-phospho-c-Myc (T58/S62) RabMAb (Cat. #AJ1192c).| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
