Application
Dot blot analysis of Phospho-NF1-S2515 Phospho-specific Pab (Cat.AP3327a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-NF1-S2515 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S2515 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NF1 Neurofibromin Neurofibromatosis-related protein NF-1 Neurofibromin truncated |
|---|---|
| Molecular Weight(MW) | 319372 Da |
| Function | Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity |
| Tissue Specificity | This Phospho-NF1-S2515 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S2515 of human NF1. |
| Entrez Gene | 4763 |
|---|
Application
Dot blot analysis of Phospho-NF1-S2515 Phospho-specific Pab (Cat.AP3327a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
