Application
Dot blot analysis of anti-Phospho-Nephrin (Y1193) antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.| Product Name | Phospho-Nephrin (Y1193) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NPHS1; NPHN; Nephrin; Renal glomerulus-specific cell adhesion receptor |
| Modification | p-Y1193 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NPHN |
|---|---|
| Alternative Names | NPHS1 NPHN Nephrin Renal glomerulus-specific cell adhesion receptor |
| Molecular Weight(MW) | 134742 Da |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity) |
| Tissue Specificity | Specifically expressed in podocytes of kidney glomeruli |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (Potential). Note=Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane |
| Entrez Gene | 4868 |
|---|---|
| Nucleotide Accession | NP_004637.1 |
Application
Dot blot analysis of anti-Phospho-Nephrin (Y1193) antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.| Application Notes | DB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
