Application
Nephrin Antibody (C-term) (Cat. #AM1865a) western blot analysis in SK-BR-3 cell line lysates (15ug/lane).This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).(1:100)Application
Confocal immunofluorescent analysis of Nephrin Antibody (C-term) (Cat#AM1865a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | Nephrin Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NPHS1; NPHN; Nephrin; Renal glomerulus-specific cell adhesion receptor |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 174CT2.1.1 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:100~200 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NPHN |
|---|---|
| Alternative Names | NPHS1 NPHN Nephrin Renal glomerulus-specific cell adhesion receptor |
| Molecular Weight(MW) | 134742 Da |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity) |
| Tissue Specificity | Specifically expressed in podocytes of kidney glomeruli |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (Potential). Note=Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane |
| Entrez Gene | 4868 |
|---|---|
| Nucleotide Accession | NP_004637.1 |
Application
Nephrin Antibody (C-term) (Cat. #AM1865a) western blot analysis in SK-BR-3 cell line lysates (15ug/lane).This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).(1:100)
Application
Confocal immunofluorescent analysis of Nephrin Antibody (C-term) (Cat#AM1865a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~200 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
