Application
Dot blot analysis of Phospho-PI3KC3-S676 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-PI3KC3-S676 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIK3C3; VPS34; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34 |
| Modification | p-S676 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | VPS34 |
|---|---|
| Alternative Names | PIK3C3 VPS34 Phosphatidylinositol 3-kinase catalytic subunit type 3 Phosphatidylinositol 3-kinase p100 subunit Phosphoinositide-3-kinase class 3 hVps34 |
| Molecular Weight(MW) | 101549 Da |
| Function | Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes Required for the abcission step in cytokinesis. Required for transport from early to late endosomes |
| Tissue Specificity | Ubiquitously expressed, with a highest expression in skeletal muscle |
| Cellular Localization | Midbody. Late endosome. |
| Entrez Gene | 5289 |
|---|
Application
Dot blot analysis of Phospho-PI3KC3-S676 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
