Application
Western blot analysis of PI3KC3 Antibody (Center K269) transiently transfected HEK-293 cell line lysate (1ug/lane). PI3KC3 (arrow) was detected using the purified Pab (1:1500 dilution).Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with Autophagy Kinase PI3KC3 antibody (Center K269), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PI3KC3 Antibody (Center K269) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIK3C3; VPS34; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:250~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | VPS34 |
|---|---|
| Alternative Names | PIK3C3 VPS34 Phosphatidylinositol 3-kinase catalytic subunit type 3 Phosphatidylinositol 3-kinase p100 subunit Phosphoinositide-3-kinase class 3 hVps34 |
| Molecular Weight(MW) | 101549 Da |
| Function | Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes Required for the abcission step in cytokinesis. Required for transport from early to late endosomes |
| Tissue Specificity | Ubiquitously expressed, with a highest expression in skeletal muscle |
| Cellular Localization | Midbody. Late endosome. |
| Entrez Gene | 5289 |
|---|
Application
Western blot analysis of PI3KC3 Antibody (Center K269) transiently transfected HEK-293 cell line lysate (1ug/lane). PI3KC3 (arrow) was detected using the purified Pab (1:1500 dilution).
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with Autophagy Kinase PI3KC3 antibody (Center K269), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:250~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
