Application
Fluorescent image of U251 cells stained with phospho-RAB24-Y17 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3393a phospho-RAB24-Y17 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Phospho- RAB24-Y17 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Application
Dot blot analysis of Phospho-RAB24-S95 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-RAB24-Y17 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB24; Ras-related protein Rab-24 |
| Modification | p-Y17 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFDB |
| IF:1:100 DB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RAB24 Ras-related protein Rab-24 |
|---|---|
| Molecular Weight(MW) | 23124 Da |
| Function | May be involved in autophagy-related processes (By similarity) |
| Tissue Specificity | This Phospho-RAB24-Y17 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 2~32 amino acids surrounding Y17 of human RAB24. Patent pending. |
| Cellular Localization | Cytoplasm, cytosol. Membrane; Lipid-anchor (By similarity). Note=Only about 20-25% is recovered in the particulate fraction |
| Entrez Gene | 53917 |
|---|
Application
Fluorescent image of U251 cells stained with phospho-RAB24-Y17 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3393a phospho-RAB24-Y17 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Phospho- RAB24-Y17 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Application
Dot blot analysis of Phospho-RAB24-S95 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | IF:1:100 DB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
