Dot blot analysis of anti-Phospho-PXN-pY118 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion)
Tissue Specificity
This Phospho-PXN-pY118 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 102~131 amino acids surrounding Y118 of human PXN.
Cellular Localization
Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex (By similarity) Note=Colocalizes with integrins at the cell periphery (By similarity)
Dot blot analysis of anti-Phospho-PXN-pY118 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application Notes
DB:1:500
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.