Application
Fluorescent confocal image of U251 cells stained with phospho-RGS19-Y143 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3398a phospho-RGS19-Y143 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Phospho-RGS19 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Application
Dot blot analysis of Phospho-RGS19-Y143 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-RGS19-Y143 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RGS19; GAIP; GNAI3IP; Regulator of G-protein signaling 19; G-alpha-interacting protein |
| Modification | p-Y143 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFDB |
| IF:1:200 DB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GAIP GNAI3IP |
|---|---|
| Alternative Names | RGS19 GAIP GNAI3IP Regulator of G-protein signaling 19 G-alpha-interacting protein |
| Molecular Weight(MW) | 24636 Da |
| Function | Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the order G(i)a3 > G(i)a1 > G(o)a >> G(z)a/G(i)a2 Activity on G(z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein |
| Tissue Specificity | Highest expression in lung. Placenta, liver and heart also express high levels of GAIP |
| Cellular Localization | Membrane; Lipid-anchor. |
| Entrez Gene | 10287 |
|---|
Application
Fluorescent confocal image of U251 cells stained with phospho-RGS19-Y143 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3398a phospho-RGS19-Y143 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Phospho-RGS19 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Application
Dot blot analysis of Phospho-RGS19-Y143 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | IF:1:200 DB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
