Application
Dot blot analysis of anti-phospho-Sox2-pS250 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
Phospho-Sox2-pS250 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Phospho-Sox2-pS250 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
Phospho-Sox2-pS250 Antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | phospho-Sox2- pS250 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SOX2; Transcription factor SOX-2 |
| Modification | p-S250 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBIHCFC |
| DB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SOX2 Transcription factor SOX-2 |
|---|---|
| Molecular Weight(MW) | 34310 Da |
| Function | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity) |
| Tissue Specificity | This phospho-Sox2-pS250 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between amino acids 230-260 surrounding S250 of human Sox2. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6657 |
|---|---|
| Nucleotide Accession | NP_003097.1 |
Application
Dot blot analysis of anti-phospho-Sox2-pS250 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
Phospho-Sox2-pS250 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Phospho-Sox2-pS250 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Phospho-Sox2-pS250 Antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | DB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
