Application
Dot blot analysis of anti-Phospho-SPRY4-pY75 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-SPRY4-pY75 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPRY4; Protein sprouty homolog 4 |
| Modification | p-Y75 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:0.388888888889 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SPRY4 Protein sprouty homolog 4 |
|---|---|
| Molecular Weight(MW) | 32541 Da |
| Function | Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 |
| Tissue Specificity | This Phospho-SPRY4-pY75 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 57~86 amino acids surrounding Y75 of human SPRY4. |
| Cellular Localization | Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side (Potential) |
| Entrez Gene | 81848 |
|---|
Application
Dot blot analysis of anti-Phospho-SPRY4-pY75 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:0.388888888889 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
