Application
Dot blot analysis of Phospho-TNIK-pS764 Pab and TNIK-pS764 Pab (AP7970d)on nitrocellulose membrane. 50ng of Phospho-peptide (BP3276a) or Non Phospho-peptide (BP7970d) per dot were adsorbed. Antobodies working concentration was 0.5ug per ml. P-Pab: phosphorylated antibody; NP-Pab: non-phophorylated antibody; P-Pep: phospho-peptide; NP-Pep: non-phospho-peptide.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Phospho-TNIK-pS764 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TNIK; KIAA0551; TRAF2 and NCK-interacting protein kinase |
| Modification | p-S764 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBIHC |
| DB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0551 |
|---|---|
| Alternative Names | TNIK KIAA0551 TRAF2 and NCK-interacting protein kinase |
| Molecular Weight(MW) | 154943 Da |
| Function | Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading Phosphorylates SMAD1 on Thr-322 |
| Tissue Specificity | Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues |
| Cellular Localization | Nucleus. Cytoplasm. Recycling endosome. Cytoplasm, cytoskeleton. Note=Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression |
| Entrez Gene | 23043 |
|---|
Application
Dot blot analysis of Phospho-TNIK-pS764 Pab and TNIK-pS764 Pab (AP7970d)on nitrocellulose membrane. 50ng of Phospho-peptide (BP3276a) or Non Phospho-peptide (BP7970d) per dot were adsorbed. Antobodies working concentration was 0.5ug per ml. P-Pab: phosphorylated antibody; NP-Pab: non-phophorylated antibody; P-Pep: phospho-peptide; NP-Pep: non-phospho-peptide.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | DB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
